产品描述
This Mouse SOCS1 ELISA Kit was designed for the quantitative measurement of Mouse SOCS1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .
别名
Socs1 ELISA Kit; Cish1 ELISA Kit; Ssi1 ELISA Kit; Suppressor of cytokine signaling 1 ELISA Kit; SOCS-1 ELISA Kit; JAK-binding protein ELISA Kit; JAB ELISA Kit; STAT-induced STAT inhibitor 1 ELISA Kit; SSI-1 ELISA Kit
缩写
SOCS-1
Uniprot No
O35716
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOCS-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 84-94 | |
| 1:2 | Average % | 98 | |
| Range % | 93-102 | |
| 1:4 | Average % | 92 | |
| Range % | 88-97 | |
| 1:8 | Average % | 95 | |
| Range % | 89-99 | |
回收率
| The recovery of mouse SOCS-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 86-95 | |
| EDTA plasma (n=4) | 99 | 94-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 2.482 | 2.452 | 2.467 | 2.328 | | | 2000 | 1.976 | 2.071 | 2.024 | 1.885 | | | 1000 | 1.604 | 1.663 | 1.634 | 1.495 | | | 500 | 1.195 | 1.139 | 1.167 | 1.028 | | | 250 | 0.624 | 0.614 | 0.619 | 0.480 | | | 125 | 0.439 | 0.416 | 0.428 | 0.289 | | | 62.5 | 0.229 | 0.246 | 0.238 | 0.099 | | | 0 | 0.141 | 0.137 | 0.139 | | |
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货期
3-5 working days