产品描述

    TNF（肿瘤坏死因子，tumor necrosis factor）是一种细胞因子，主要由巨噬细胞、T淋巴细胞、自然杀伤细胞等产生。它具有多种生物学功能，包括细胞凋亡、炎症、免疫调节和细胞增殖等。TNF通过激活多种信号转导通路，介导宿主对细菌、病毒和其他致病微生物的免疫应答，同时也对肿瘤的发生、发展有重要作用。

别名

TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit

缩写

TNF

Uniprot No

Q06599

灵敏度

0.05 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of bovine TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		88-101
1:2		Average %		92
		Range %		85-99
1:4		Average %		90
		Range %		85-94
1:8		Average %		96
		Range %		90-100

回收率

The recovery of bovine TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		94-101
EDTA plasma (n=4)		95		89-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average     20 0.173 0.176 0.175     5 0.312 0.325 0.319     1.6 0.617 0.634 0.626     0.4 1.035 1.118 1.077     0.1 1.396 1.405 1.401     0 2.513 2.546 2.530

货期

3-5 working days