产品描述

This Human PDGFRB ELISA Kit was designed for the quantitative measurement of Human PDGFRB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

别名

Beta platelet derived growth factor receptor ELISA Kit; Beta-type platelet-derived growth factor receptor ELISA Kit; CD 140B ELISA Kit; CD140 antigen-like family member B ELISA Kit; CD140b ELISA Kit; CD140b antigen ELISA Kit; IBGC4 ELISA Kit; IMF1 ELISA Kit; JTK12 ELISA Kit; OTTHUMP00000160528 ELISA Kit; PDGF R beta ELISA Kit; PDGF Receptor beta ELISA Kit; PDGF-R-beta ELISA Kit; PDGFR 1 ELISA Kit; PDGFR ELISA Kit; PDGFR beta ELISA Kit; PDGFR1 ELISA Kit; PDGFRB ELISA Kit; PGFRB_HUMAN ELISA Kit; Platelet derived growth factor receptor 1 ELISA Kit; Platelet derived growth factor receptor beta ELISA Kit; Platelet derived growth factor receptor beta polypeptide ELISA Kit

缩写

PDGFRB

Uniprot No

P09619

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PDGFRB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		84-91
1:2		Average %		102
		Range %		98-105
1:4		Average %		91
		Range %		85-94
1:8		Average %		94
		Range %		90-98

回收率

The recovery of human PDGFRB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		88-97
EDTA plasma (n=4)		108		102-113

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 4000 2.575 2.521 2.548 2.371 2000 2.128 2.184 2.156 1.979 1000 1.675 1.586 1.631 1.454 500 1.067 1.047 1.057 0.880 250 0.684 0.678 0.681 0.504 125 0.468 0.498 0.483 0.306 62.5 0.292 0.287 0.290 0.113 0 0.179 0.175 0.177

货期

3-5 working days