产品描述
This Human PRF1 ELISA Kit was designed for the quantitative measurement of Human PRF1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Cytolysin ELISA Kit; FLH2 ELISA Kit; HPLH2 ELISA Kit; Lymphocyte pore-forming protein ELISA Kit; P1 ELISA Kit; PERF_HUMAN ELISA Kit; perforin 1 (pore forming protein) ELISA Kit; Perforin 1 ELISA Kit; Perforin-1 ELISA Kit; PFP ELISA Kit; PGFL ELISA Kit; PIGF ELISA Kit; PIGF-2 ELISA Kit; PLGF ELISA Kit; Pore forming protein ELISA Kit; prf1 ELISA Kit; SHGC-10760 ELISA Kit
缩写
PRF1
Uniprot No
P14222
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 91-100 | |
| 1:2 | Average % | 100 | |
| Range % | 95-104 | |
| 1:4 | Average % | 92 | |
| Range % | 85-95 | |
| 1:8 | Average % | 99 | |
| Range % | 92-103 | |
回收率
| The recovery of human PRF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 85-96 | |
| EDTA plasma (n=4) | 90 | 82-94 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.764 | 2.666 | 2.715 | 2.602 | | | 10 | 1.912 | 1.932 | 1.922 | 1.809 | | | 5 | 1.301 | 1.313 | 1.307 | 1.194 | | | 2.5 | 0.689 | 0.657 | 0.673 | 0.560 | | | 1.25 | 0.376 | 0.358 | 0.367 | 0.254 | | | 0.63 | 0.281 | 0.272 | 0.277 | 0.164 | | | 0.312 | 0.189 | 0.184 | 0.187 | 0.074 | | | 0 | 0.114 | 0.112 | 0.113 | | |
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货期
3-5 working days