产品描述
This Pig TIMP1 ELISA Kit was designed for the quantitative measurement of Pig TIMP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.
别名
TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
缩写
TIMP1
Uniprot No
P35624
灵敏度
1.95 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 89-101 | |
| 1:2 | Average % | 89 | |
| Range % | 83-98 | |
| 1:4 | Average % | 92 | |
| Range % | 86-105 | |
| 1:8 | Average % | 94 | |
| Range % | 89-107 | |
回收率
| The recovery of pig TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 85-99 | |
| EDTA plasma (n=4) | 97 | 92-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.422 | 2.395 | 2.409 | 2.236 | | | 250 | 1.466 | 1.506 | 1.486 | 1.313 | | | 125 | 0.986 | 1.007 | 0.997 | 0.824 | | | 62.5 | 0.611 | 0.589 | 0.600 | 0.427 | | | 31.2 | 0.425 | 0.454 | 0.440 | 0.267 | | | 15.6 | 0.342 | 0.362 | 0.352 | 0.179 | | | 7.8 | 0.242 | 0.250 | 0.246 | 0.073 | | | 0 | 0.175 | 0.170 | 0.173 | | |
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货期
3-5 working days