产品描述

This Human POMC ELISA Kit was designed for the quantitative measurement of Human POMC protein in serum, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL .

别名

ACTH ELISA Kit; Adrenocorticotropic hormone ELISA Kit; Adrenocorticotropin ELISA Kit; alpha melanocyte stimulating hormone ELISA Kit; Alpha MSH ELISA Kit; Alpha-MSH ELISA Kit; beta endorphin ELISA Kit; Beta LPH ELISA Kit; beta melanocyte stimulating hormone ELISA Kit; Beta MSH ELISA Kit; Beta-LPH ELISA Kit; Beta-MSH ELISA Kit; CLIP ELISA Kit; COLI_HUMAN ELISA Kit; Corticotropin like intermediary peptide ELISA Kit; Corticotropin lipotropin ELISA Kit; Corticotropin-lipotropin ELISA Kit; Gamma MSH ELISA Kit; Gamma-LPH ELISA Kit; Gamma-MSH ELISA Kit; Lipotropin beta ELISA Kit; Lipotropin gamma ELISA Kit; LPH ELISA Kit; Melanotropin alpha ELISA Kit; Melanotropin ELISA Kit; Melanotropin beta ELISA Kit; Melanotropin gamma ELISA Kit; met enkephalin ELISA Kit; Met-enkephalin ELISA Kit; MSH ELISA Kit; NPP ELISA Kit; POC ELISA Kit; POMC ELISA Kit; pro ACTH endorphin ELISA Kit; Proopiomelanocortin ELISA Kit; Proopiomelanocortin preproprotein ELISA Kit

缩写

POMC

Uniprot No

P01189

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		90-102
1:2		Average %		89
		Range %		84-95
1:4		Average %		95
		Range %		90-100
1:8		Average %		92
		Range %		87-102

回收率

The recovery of human POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-96
EDTA plasma (n=4)		102		98-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 5000 2.865 2.899 2.882 2.705 2500 2.498 2.486 2.492 2.315 1250 2.004 2.056 2.030 1.853 625 1.562 1.598 1.580 1.403 312 1.084 1.054 1.069 0.892 156 0.692 0.682 0.687 0.510 78 0.324 0.357 0.341 0.164 0 0.174 0.179 0.177

货期

3-5 working days