产品描述

This Human RORC ELISA Kit was designed for the quantitative measurement of Human RORC protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

别名

IMD42 ELISA Kit; MGC129539 ELISA Kit; NR1F3 ELISA Kit; Nuclear receptor ROR gamma ELISA Kit; Nuclear receptor ROR-gamma ELISA Kit; Nuclear receptor RZR gamma ELISA Kit; Nuclear receptor RZR-gamma ELISA Kit; Nuclear receptor subfamily 1 group F member 3 ELISA Kit; RAR related orphan nuclear receptor variant 2 ELISA Kit; RAR related orphan receptor C ELISA Kit; RAR related orphan receptor C, isoform a ELISA Kit; RAR related orphan receptor gamma ELISA Kit; RAR-related orphan receptor C ELISA Kit; Retinoic acid binding receptor gamma ELISA Kit; Retinoid related orphan receptor gamma ELISA Kit; Retinoid-related orphan receptor-gamma ELISA Kit; Rorc ELISA Kit; RORG ELISA Kit; RORG_HUMAN ELISA Kit; RZR GAMMA ELISA Kit; RZRG ELISA Kit; TOR ELISA Kit

缩写

RORC

Uniprot No

P51449

灵敏度

3.12 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human RORC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-96
1:2		Average %		98
		Range %		93-103
1:4		Average %		84
		Range %		81-87
1:8		Average %		88
		Range %		83-93

回收率

The recovery of human RORC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		102		97-107
EDTA plasma (n=4)		92		89-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 800 2.424 2.235 2.330 2.202 400 1.670 1.705 1.688 1.560 200 1.049 1.055 1.052 0.924 100 0.664 0.704 0.684 0.556 50 0.432 0.399 0.416 0.288 25 0.291 0.286 0.289 0.161 12.5 0.215 0.201 0.208 0.080 0 0.125 0.130 0.128

货期

3-5 working days