产品描述
This Mouse PDGFRA ELISA Kit was designed for the quantitative measurement of Mouse PDGFRA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Pdgfra ELISA Kit; Platelet-derived growth factor receptor alpha ELISA Kit; PDGF-R-alpha ELISA Kit; PDGFR-alpha ELISA Kit; EC 2.7.10.1 ELISA Kit; Alpha platelet-derived growth factor receptor ELISA Kit; Alpha-type platelet-derived growth factor receptor ELISA Kit; CD140 antigen-like family member A ELISA Kit; Platelet-derived growth factor alpha receptor ELISA Kit; CD antigen CD140a ELISA Kit
缩写
PDGFRA
Uniprot No
P26618
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGFsR-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 86-93 | |
| 1:2 | Average % | 97 | |
| Range % | 92-101 | |
| 1:4 | Average % | 91 | |
| Range % | 88-97 | |
| 1:8 | Average % | 92 | |
| Range % | 84-96 | |
回收率
| The recovery of mouse PDGFsR-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 88-98 | |
| EDTA plasma (n=4) | 99 | 96-105 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.795 | 3.012 | 2.904 | 2.787 | | | 500 | 2.214 | 2.304 | 2.259 | 2.142 | | | 250 | 1.436 | 1.550 | 1.493 | 1.376 | | | 125 | 0.899 | 0.974 | 0.937 | 0.820 | | | 62.5 | 0.472 | 0.461 | 0.467 | 0.350 | | | 31.25 | 0.351 | 0.342 | 0.347 | 0.230 | | | 15.6 | 0.244 | 0.253 | 0.249 | 0.132 | | | 0 | 0.113 | 0.120 | 0.117 | | |
|
货期
3-5 working days