产品描述
This Human SIGLEC8 ELISA Kit was designed for the quantitative measurement of Human SIGLEC8 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
CD329 antigen ELISA Kit; CDw329 ELISA Kit; SAF 2 ELISA Kit; SAF-2 ELISA Kit; SAF2 ELISA Kit; Sialic acid binding Ig like lectin 8 ELISA Kit; Sialic acid-binding Ig-like lectin 8 ELISA Kit; Sialoadhesin family member 2 ELISA Kit; SIGL8_HUMAN ELISA Kit; Siglec 8 ELISA Kit; Siglec-8 ELISA Kit; SIGLEC8 ELISA Kit; SIGLEC8L ELISA Kit
缩写
SIGLEC8
Uniprot No
Q9NYZ4
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SIGLEC8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 100 | | |
| Range % | 95-107 | | |
| 1:2 | Average % | 96 | | |
| Range % | 92-100 | | |
| 1:4 | Average % | 84 | | |
| Range % | 80-92 | | |
| 1:8 | Average % | 88 | | |
| Range % | 84-92 | | |
回收率
| The recovery of human SIGLEC8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 94 | 90-100 | | |
| EDTA plasma (n=4) | 90 | 85-97 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 10 | 2.164 | 2.246 | 2.205 | 2.040 | | | | 5 | 1.618 | 1.634 | 1.626 | 1.461 | | | | 2.5 | 1.201 | 1.175 | 1.188 | 1.023 | | | | 1.25 | 0.792 | 0.823 | 0.808 | 0.643 | | | | 0.625 | 0.558 | 0.571 | 0.565 | 0.400 | | | | 0.312 | 0.419 | 0.442 | 0.431 | 0.266 | | | | 0.156 | 0.324 | 0.305 | 0.315 | 0.150 | | | | 0 | 0.162 | 0.167 | 0.165 | | | |
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货期
3-5 working days