产品描述
CUSABIO's SARS-CoV-2 Neutralizing Antibody ELISA Kit is intended for qualitative measurement of neutralizing antibodies in human serum or plasma. SARS-CoV-2 neutralizing antibodies directly target the SARS-CoV-2 S receptor-binding domain (RBD), blocking the protein's interaction with the host ACE2 receptor, thus interfering with the viral attachment to the cell as well as its entry into the cell. When using the reference neutralizing antibody provided in the kit, the assay has a detection range of 9.75-10000 ng/ml, a sensitivity of as low as 9.75 ng/ml, and Intra- /Inter-assay Precision low than 15%. This detection kit is simple in operation and scalable in sample size (high throughput). Multiple validations display it has excellent sensitivity and high specificity. It is conducive to identify individuals with an adaptive immune response to SARS-CoV-2, indicating recent or prior infection. And it should not be used for the diagnosis of acute SARS-CoV-2 infection.
别名
2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2
缩写
SARS-CoV-2 neutralizing Ab
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Infectious Diseases
测定原理
qualitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
| Inter-assay Precision (Precision between assays): CV%<15% |
| Three samples of known concentration were tested in twenty assays to assess. |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 |
| ng/ml |
OD1 |
OD2 |
Average |
| 10000 |
0.099 |
0.092 |
0.096 |
| 5000 |
0.145 |
0.136 |
0.141 |
| 2500 |
0.194 |
0.183 |
0.189 |
| 1250 |
0.274 |
0.263 |
0.269 |
| 625 |
0.344 |
0.371 |
0.358 |
| 312 |
0.519 |
0.528 |
0.524 |
| 156 |
0.901 |
0.907 |
0.904 |
| 78 |
1.509 |
1.412 |
1.461 |
| 39 |
1.787 |
1.723 |
1.755 |
| 19.5 |
2.121 |
1.958 |
2.040 |
| 9.75 |
2.233 |
2.138 |
2.186 |
| 0 |
2.394 |
2.328 |
2.361 |
货期
3-5 working days
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