产品描述

This Human PI3K ELISA Kit was designed for the quantitative measurement of Human PI3K protein in serum, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA Kit; 5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit; caPI3K ELISA Kit; CLOVE ELISA Kit; CWS5 ELISA Kit; MCAP ELISA Kit; MCM ELISA Kit; MCMTC ELISA Kit; MGC142161 ELISA Kit; MGC142163 ELISA Kit; p110 alpha ELISA Kit; p110alpha ELISA Kit; Phosphatidylinositol 3 kinase catalytic alpha polypeptide ELISA Kit; Phosphatidylinositol 3 kinase catalytic 110 KD alpha ELISA Kit; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha ELISA Kit; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform ELISA Kit; Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha ELISA Kit; Phosphatidylinositol-4 ELISA Kit; Phosphoinositide 3 kinase catalytic alpha polypeptide ELISA Kit; PI 3 Kinase catalytic subunit alpha ELISA Kit; PI3 kinase p110 subunit alpha ELISA Kit; PI3-kinase subunit alpha ELISA Kit; PI3K ELISA Kit; PI3K-alpha ELISA Kit; PI3KC A ELISA Kit; PIK3C A ELISA Kit; Pik3ca ELISA Kit; PK3CA ELISA Kit; PK3CA_HUMAN ELISA Kit; PtdIns 3 kinase p110 ELISA Kit; PtdIns-3-kinase subunit alpha ELISA Kit; PtdIns-3-kinase subunit p110-alpha ELISA Kit; Serine/threonine protein kinase PIK3CA ELISA Kit

缩写

PI3K

Uniprot No

P42336

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		84-98
1:2		Average %		98
		Range %		91-105
1:4		Average %		92
		Range %		85-99
1:8		Average %		86
		Range %		80-92

回收率

The recovery of human PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		92-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.942 2.900 2.921 2.827 20 2.289 2.278 2.284 2.190 10 1.576 1.545 1.561 1.467 5 0.788 0.768 0.778 0.684 2.5 0.472 0.445 0.459 0.365 1.25 0.293 0.278 0.286 0.192 0.625 0.218 0.205 0.212 0.118 0 0.096 0.092 0.094

货期

3-5 working days