产品描述
PF4是一种负电荷的蛋白质,由血小板和内皮细胞合成,可以结合和中和肝素。它在血管内皮损伤后释放,并与凝血、炎症、免疫反应等过程密切相关。PF4的含量异常可以反映出许多疾病的存在或发展。因此,检测PF4的水平可以为诊断、监测疾病提供重要的帮助。
华美生物所提供的Mouse Platelet Factor 4,PF-4 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的PF4,其灵敏度为1.603 ng/mL,检测范围为3.125 ng/mL-200 ng/mL。
别名
Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit
缩写
PF4
Uniprot No
Q9Z126
灵敏度
1.603 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
86 |
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| Range % |
81-90 |
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| 1:400 |
Average % |
98 |
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| Range % |
92-104 |
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| 1:800 |
Average % |
97 |
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| Range % |
94-102 |
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| 1:1600 |
Average % |
94 |
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| Range % |
89-98 |
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回收率
| The recovery of mouse PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
86-94 |
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| EDTA plasma (n=4) |
98 |
92-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.501 |
2.402 |
2.452 |
2.308 |
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| 100 |
2.093 |
1.975 |
2.034 |
1.890 |
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| 50 |
1.354 |
1.383 |
1.369 |
1.225 |
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| 25 |
0.778 |
0.769 |
0.774 |
0.630 |
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| 12.5 |
0.386 |
0.371 |
0.379 |
0.235 |
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| 6.25 |
0.268 |
0.257 |
0.263 |
0.119 |
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| 3.125 |
0.209 |
0.202 |
0.206 |
0.062 |
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| 0 |
0.144 |
0.143 |
0.144 |
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货期
3-5 working days