产品描述

This Human PRDX6 ELISA Kit was designed for the quantitative measurement of Human PRDX6 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

别名

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缩写

PRDX6

Uniprot No

P30041

灵敏度

3.12 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-87
1:2		Average %		92
		Range %		87-96
1:4		Average %		91
		Range %		85-97
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human PRDX6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		95-105
EDTA plasma (n=4)		97		93-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 800 2.144 2.302 2.223 2.098 400 1.718 1.768 1.743 1.618 200 1.303 1.321 1.312 1.187 100 0.847 0.868 0.858 0.733 50 0.535 0.561 0.548 0.423 25 0.352 0.342 0.347 0.222 12.5 0.286 0.289 0.288 0.163 0 0.124 0.126 0.125

货期

3-5 working days