产品描述
This Rat PPARA ELISA Kit was designed for the quantitative measurement of Rat PPARA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Ppara ELISA Kit; Nr1c1 ELISA Kit; Ppar ELISA Kit; Peroxisome proliferator-activated receptor alpha ELISA Kit; PPAR-alpha ELISA Kit; Nuclear receptor subfamily 1 group C member 1 ELISA Kit
缩写
PPARA
Uniprot No
P37230
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PPAR-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 89-100 | |
| 1:2 | Average % | 94 | |
| Range % | 88-98 | |
| 1:4 | Average % | 96 | |
| Range % | 91-100 | |
| 1:8 | Average % | 95 | |
| Range % | 87-101 | |
回收率
| The recovery of rat PPAR-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 86-96 | |
| EDTA plasma (n=4) | 90 | 85-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.183 | 2.177 | 2.180 | 2.068 | | | 10 | 1.654 | 1.627 | 1.641 | 1.529 | | | 5 | 1.240 | 1.211 | 1.226 | 1.114 | | | 2.5 | 0.859 | 0.842 | 0.851 | 0.739 | | | 1.25 | 0.546 | 0.513 | 0.530 | 0.418 | | | 0.625 | 0.381 | 0.373 | 0.377 | 0.265 | | | 0.312 | 0.166 | 0.157 | 0.162 | 0.050 | | | 0 | 0.111 | 0.113 | 0.112 | | |
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货期
3-5 working days