产品描述
This Mouse PECAM1 ELISA Kit was designed for the quantitative measurement of Mouse PECAM1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
Pecam1 ELISA Kit; Pecam ELISA Kit; Pecam-1 ELISA Kit; Platelet endothelial cell adhesion molecule ELISA Kit; PECAM-1 ELISA Kit; CD antigen CD31 ELISA Kit
缩写
PECAM1
Uniprot No
Q08481
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse sPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:100 | Average % | 87 | | |
| Range % | 82-91 | | |
| 1:200 | Average % | 96 | | |
| Range % | 92-100 | | |
| 1:400 | Average % | 104 | | |
| Range % | 100-110 | | |
| 1:800 | Average % | 90 | | |
| Range % | 85-94 | | |
回收率
| The recovery of mouse sPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 88-96 | | |
| EDTA plasma (n=4) | 84 | 80-88 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 200 | 2.923 | 2.814 | 2.869 | 2.771 | | | | 100 | 2.059 | 2.148 | 2.104 | 2.006 | | | | 50 | 1.239 | 1.159 | 1.199 | 1.101 | | | | 25 | 0.687 | 0.697 | 0.692 | 0.594 | | | | 12.5 | 0.419 | 0.405 | 0.412 | 0.314 | | | | 6.25 | 0.281 | 0.273 | 0.277 | 0.179 | | | | 3.12 | 0.184 | 0.186 | 0.185 | 0.087 | | | | 0 | 0.095 | 0.101 | 0.098 | | | |
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货期
3-5 working days