产品描述

This Human TRAF6 ELISA Kit was designed for the quantitative measurement of Human TRAF6 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.8 pg/mL.

别名

E3 ubiquitin-protein ligase TRAF6 ELISA Kit; Interleukin 1 signal transducer ELISA Kit; Interleukin-1 signal transducer ELISA Kit; MGC 3310 ELISA Kit; MGC:3310 ELISA Kit; MGC3310 ELISA Kit; OTTHUMP00000232772 ELISA Kit; OTTHUMP00000232773 ELISA Kit; RING finger protein 85 ELISA Kit; RNF 85 ELISA Kit; RNF85 ELISA Kit; TNF receptor associated factor 6 ELISA Kit; TNF receptor-associated factor 6 ELISA Kit; TNF receptor-associated factor 6; E3 ubiquitin protein ligase ELISA Kit; TRAF 6 ELISA Kit; Traf6 ELISA Kit; TRAF6_HUMAN ELISA Kit

缩写

TRAF6

Uniprot No

Q9Y4K3

灵敏度

9.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TRAF6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-99
1:2		Average %		103
		Range %		99-104
1:4		Average %		93
		Range %		87-98
1:8		Average %		91
		Range %		89-97

回收率

The recovery of human TRAF6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		92-99
EDTA plasma (n=4)		90		86-93

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2500 2.444 2.246 2.345 2.239 1250 1.937 1.780 1.859 1.753 625 1.432 1.544 1.488 1.382 312.5 1.078 1.159 1.119 1.013 156 0.726 0.760 0.743 0.637 78 0.376 0.358 0.367 0.261 39 0.261 0.244 0.253 0.147 0 0.103 0.109 0.106

货期

3-5 working days