产品描述

This Human TLR4 ELISA Kit was designed for the quantitative measurement of Human TLR4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

ARMD10 ELISA Kit; CD284 ELISA Kit; CD284 antigen ELISA Kit; ESOP 1 ELISA Kit; ESOP1 ELISA Kit; Homolog of Drosophila toll ELISA Kit; hToll ELISA Kit; LY 96 ELISA Kit; LY96 ELISA Kit; Lymphocyte antigen 96 ELISA Kit; md 2 ELISA Kit; MD 2 protein ELISA Kit; MD2 protein ELISA Kit; Myeloid differentiation protein 2 ELISA Kit; Protein MD 2 ELISA Kit; Protein MD2 ELISA Kit; TLR 4 ELISA Kit; TLR4 ELISA Kit; TLR4_HUMAN ELISA Kit; TOLL ELISA Kit; Toll like receptor 4 ELISA Kit; Toll-like receptor 4 ELISA Kit

缩写

TLR4

Uniprot No

O00206

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TLR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		85
		Range %		80-93
1:10		Average %		95
		Range %		89-100
1:20		Average %		97
		Range %		93-104
1:40		Average %		95
		Range %		91-99

回收率

The recovery of human TLR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		89-103
EDTA plasma (n=4)		97		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.463 2.547 2.505 2.372 5 2.121 2.172 2.147 2.014 2.5 1.540 1.516 1.528 1.395 1.25 0.725 0.747 0.736 0.603 0.625 0.418 0.436 0.427 0.294 0.312 0.236 0.241 0.239 0.106 0.156 0.199 0.206 0.203 0.070 0 0.132 0.134 0.133

货期

3-5 working days