产品描述
VA(Vitronectin)是一种在体内广泛存在的糖蛋白,与许多生物学过程有关,如凝血、细胞外基质的重构以及炎症反应等。VA可以结合到细胞表面上的特定受体中,参与了许多信号通路的调控。在一些疾病中,VA的含量和活性也发生了改变,如某些癌症和心血管疾病
华美生物所提供的Bovine Vitamin A (VA) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测牛血清、血浆、组织匀浆样本中的VA,其灵敏度为4.672 ng/mL,检测范围为6.25 ng/mL-100 ng/mL。
缩写
VA
灵敏度
4.672 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine VA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:10 |
Average % |
95 |
|
| Range % |
91-99 |
|
| 1:20 |
Average % |
103 |
|
| Range % |
99-107 |
|
| 1:40 |
Average % |
98 |
|
| Range % |
93-103 |
|
| 1:80 |
Average % |
92 |
|
| Range % |
89-95 |
|
回收率
| The recovery of bovine VA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
99 |
93-104 |
|
| EDTA plasma (n=4) |
89 |
83-95 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
|
|
| 100 |
0.098 |
0.095 |
0.097 |
|
|
| 50 |
0.150 |
0.163 |
0.157 |
|
|
| 25 |
0.263 |
0.268 |
0.266 |
|
|
| 12.5 |
0.551 |
0.565 |
0.558 |
|
|
| 6.25 |
1.037 |
1.065 |
1.051 |
|
|
| 0 |
2.266 |
2.287 |
2.277 |
|
|
|
货期
3-5 working days