产品描述
This Mouse PDGFC ELISA Kit was designed for the quantitative measurement of Mouse PDGFC protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
别名
Pdgfc ELISA Kit; Scdgf ELISA Kit; Platelet-derived growth factor C ELISA Kit; PDGF-C ELISA Kit; Fallotein ELISA Kit; Spinal cord-derived growth factor ELISA Kit; SCDGF ELISA Kit; VEGF-E) [Cleaved into: Platelet-derived growth factor C ELISA Kit; latent form ELISA Kit; PDGFC latent form); Platelet-derived growth factor C ELISA Kit; receptor-binding form ELISA Kit; PDGFC receptor-binding form)] ELISA Kit
缩写
PDGFC
Uniprot No
Q8CI19
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 101 | | |
| Range % | 95-105 | | |
| 1:2 | Average % | 95 | | |
| Range % | 89-100 | | |
| 1:4 | Average % | 96 | | |
| Range % | 88-101 | | |
| 1:8 | Average % | 94 | | |
| Range % | 90-99 | | |
回收率
| The recovery of mouse PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 89 | 84-93 | | |
| EDTA plasma (n=4) | 98 | 90-103 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 4000 | 2.480 | 2.411 | 2.446 | 2.282 | | | | 2000 | 1.879 | 1.858 | 1.869 | 1.705 | | | | 1000 | 1.151 | 1.109 | 1.130 | 0.966 | | | | 500 | 0.636 | 0.625 | 0.631 | 0.467 | | | | 250 | 0.399 | 0.389 | 0.394 | 0.230 | | | | 125 | 0.287 | 0.278 | 0.283 | 0.119 | | | | 62.5 | 0.215 | 0.210 | 0.213 | 0.049 | | | | 0 | 0.166 | 0.162 | 0.164 | | | |
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货期
3-5 working days