产品描述
This Human PGK1 ELISA Kit was designed for the quantitative measurement of Human PGK1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL.
别名
Cell migration-inducing gene 10 protein ELISA Kit; Epididymis secretory sperm binding protein Li 68p ELISA Kit; HEL S 68p ELISA Kit; MGC117307 ELISA Kit; MGC8947 ELISA Kit; MIG10 ELISA Kit; pgk1 ELISA Kit; PGK1_HUMAN ELISA Kit; PGKA ELISA Kit; Phosphoglycerate kinase 1 ELISA Kit; Primer recognition protein 2 ELISA Kit; PRP 2 ELISA Kit
缩写
PGK1
Uniprot No
P00558
灵敏度
7 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 87-97 | |
| 1:2 | Average % | 99 | |
| Range % | 95-104 | |
| 1:4 | Average % | 101 | |
| Range % | 94-105 | |
| 1:8 | Average % | 95 | |
| Range % | 89-99 | |
回收率
| The recovery of human PGK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 86-97 | |
| EDTA plasma (n=4) | 99 | 95-107 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1800 | 2.894 | 3.011 | 2.953 | 2.762 | | | 900 | 2.266 | 2.244 | 2.255 | 2.064 | | | 450 | 1.393 | 1.474 | 1.434 | 1.243 | | | 225 | 0.734 | 0.763 | 0.749 | 0.558 | | | 112.5 | 0.497 | 0.480 | 0.489 | 0.298 | | | 56 | 0.354 | 0.338 | 0.346 | 0.155 | | | 28 | 0.267 | 0.277 | 0.272 | 0.081 | | | 0 | 0.186 | 0.195 | 0.191 | | |
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货期
3-5 working days