产品描述

This Human SIRT6 ELISA Kit was designed for the quantitative measurement of Human SIRT6 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

别名

2810449N18Rik ELISA Kit; AI043036 ELISA Kit; Mono ADP ribosyltransferase sirtuin 6 ELISA Kit; NAD-dependent protein deacetylase sirtuin-6 ELISA Kit; Regulatory protein SIR2 homolog 6 ELISA Kit; Regulatory protein SIR2 homolog ELISA Kit; SIR2 like 6 ELISA Kit; SIR2 like protein 6 ELISA Kit; Sir2 related protein type 6 ELISA Kit; SIR2-like protein 6 ELISA Kit; SIR2L6 ELISA Kit; SIR6_HUMAN ELISA Kit; SIRT 6 ELISA Kit; SIRT6 ELISA Kit; Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) ELISA Kit; Sirtuin 6 ELISA Kit; Sirtuin type 6 ELISA Kit; Sirtuin6 ELISA Kit

缩写

SIRT6

Uniprot No

Q8N6T7

灵敏度

0.78 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SIRT6/SIR2L6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		99
		Range %		94-104
1:200		Average %		103
		Range %		99-107
1:400		Average %		89
		Range %		85-95
1:800		Average %		96
		Range %		93-99

回收率

The recovery of human SIRT6/SIR2L6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		88-98
EDTA plasma (n=4)		103		99-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 200 2.568 2.689 2.629 2.512 100 1.835 1.965 1.900 1.783 50 1.389 1.321 1.355 1.238 25 0.866 0.842 0.854 0.737 12.5 0.574 0.535 0.555 0.438 6.25 0.322 0.335 0.329 0.212 3.12 0.209 0.204 0.207 0.090 0 0.117 0.116 0.117

货期

3-5 working days