产品描述
This rat polypeptide YY (PYY) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat PYY in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PYY bound in the initial step.
PYY is an appetite-inhibiting gut hormone secreted from the L-cells of the gastrointestinal tract. Low levels of PYY are associated with higher BMI and obesity. Circulating PYY increases satiety and subsequently reduces food intake through gut-brain communication, and suppresses gastrointestinal motility and pancreatic hormone secretion. Circulating PYY levels increase during aerobic exercise. Additionally, PYY plays an integral part in maintaining energy homeostasis. PYY also plays an important role in the absorption of water and electrolytes.
别名
PyyPeptide YY ELISA kit; PYY ELISA kit; Peptide tyrosine tyrosine) [Cleaved into: Peptide YY(3-36 ELISA kit; PYY-II)] ELISA kit
缩写
PYY
Uniprot No
P10631
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 90-100 | |
| 1:2 | Average % | 88 | |
| Range % | 84-93 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 99 | |
| Range % | 94-105 | |
回收率
| The recovery of rat PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 92-101 | |
| EDTA plasma (n=4) | 100 | 95-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.478 | 2.374 | 2.426 | 2.318 | | | 250 | 1.754 | 1.852 | 1.803 | 1.695 | | | 125 | 1.188 | 1.226 | 1.207 | 1.099 | | | 62.5 | 0.725 | 0.689 | 0.707 | 0.599 | | | 31.2 | 0.403 | 0.382 | 0.393 | 0.285 | | | 15.6 | 0.272 | 0.257 | 0.265 | 0.157 | | | 7.8 | 0.189 | 0.178 | 0.184 | 0.076 | | | 0 | 0.110 | 0.105 | 0.108 | | |
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货期
3-5 working days
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