产品描述

This human NF-κB p65 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human NF-κB p65 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of NF-κB p65 bound in the initial step.

p65, also known as RELA, is one of the five components that form the NF-κB transcription factor family and plays a central role in inflammation and immunity. p65 dimerizes with NF-κB1 (p50) to form the heterodimer NF-κB, which is involved in multiple physiological and pathological gene regulation processes, such as inflammatory response, immune response, cell division, proliferation, and apoptosis. Studies have shown that the expression of NF-κB P65 in liver cancer tissues is higher than that in adjacent tissues, and the key to regulating the function of NF-κB is to inhibit the promoting effect of P65 on liver tumors. It is speculated that blocking the activity of NF-κB P65 can inhibit the differentiation and growth of tumor cells, providing a new idea for clinical treatment.

别名

Avian reticuloendotheliosis viral (v rel) oncogene homolog A ELISA Kit; MGC131774 ELISA Kit; NF kappa B p65delta3 ELISA Kit; nfkappabp65 ELISA Kit; NFkB p65 ELISA Kit; NFKB3 ELISA Kit; Nuclear factor kappaB ELISA Kit; Nuclear Factor NF Kappa B p65 Subunit ELISA Kit; Nuclear factor NF-kappa-B p65 subunit ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 ELISA Kit; OTTHUMP00000233473 ELISA Kit; OTTHUMP00000233474 ELISA Kit; OTTHUMP00000233475 ELISA Kit; OTTHUMP00000233476 ELISA Kit; OTTHUMP00000233900 ELISA Kit; p65 ELISA Kit; p65 NF kappaB ELISA Kit; p65 NFkB ELISA Kit; relA ELISA Kit; TF65_HUMAN ELISA Kit; Transcription factor NFKB3 ELISA Kit; Transcription factor p65 ELISA Kit; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) ELISA Kit; V rel avian reticuloendotheliosis viral oncogene homolog A ELISA Kit; v rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit; V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 ELISA Kit

缩写

RELA

Uniprot No

Q04206

灵敏度

11.75 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		83-92
1:2		Average %		101
		Range %		95-104
1:4		Average %		102
		Range %		98-105
1:8		Average %		95
		Range %		90-98

回收率

The recovery of human NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-96
EDTA plasma (n=4)		95		91-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 3000 2.751 2.732 2.742 2.618 1500 1.966 1.876 1.921 1.797 750 1.356 1.344 1.350 1.226 375 0.790 0.777 0.784 0.660 187.5 0.421 0.403 0.412 0.288 94 0.323 0.312 0.318 0.194 47 0.209 0.196 0.203 0.079 0 0.125 0.123 0.124

货期

3-5 working days