产品描述
This Human S100A8/A9 ELISA Kit was designed for the quantitative measurement of Human S100A8/A9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.
别名
N/A
缩写
S100A8/A9
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8/A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 80-92 | |
| 1:2 | Average % | 95 | |
| Range % | 91-98 | |
| 1:4 | Average % | 92 | |
| Range % | 87-98 | |
| 1:8 | Average % | 95 | |
| Range % | 90-103 | |
回收率
| The recovery of human S100A8/A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 85 | 81-89 | |
| EDTA plasma (n=4) | 92 | 85-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.545 | 2.541 | 2.543 | 2.461 | | | 50 | 1.850 | 1.726 | 1.788 | 1.706 | | | 25 | 1.021 | 1.001 | 1.011 | 0.929 | | | 12.5 | 0.583 | 0.567 | 0.575 | 0.493 | | | 6.25 | 0.357 | 0.334 | 0.346 | 0.264 | | | 3.12 | 0.205 | 0.198 | 0.202 | 0.120 | | | 1.56 | 0.139 | 0.133 | 0.136 | 0.054 | | | 0 | 0.085 | 0.079 | 0.082 | | |
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货期
3-5 working days