产品描述
This Human Pro-PSA ELISA Kit was designed for the quantitative measurement of Human Pro-PSA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-4000 pg/mL and the sensitivity is 62.5 pg/mL.
缩写
Pro-PSA
灵敏度
62.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<20% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-PSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 89-99 | |
| 1:2 | Average % | 90 | |
| Range % | 84-94 | |
| 1:4 | Average % | 105 | |
| Range % | 98-108 | |
| 1:8 | Average % | 92 | |
| Range % | 88-96 | |
回收率
| The recovery of human Pro-PSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 83-94 | |
| EDTA plasma (n=4) | 93 | 89-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | | | | 4000 | 2.351 | 2.201 | 2.276 | | | | 2000 | 1.412 | 1.356 | 1.384 | | | | 1000 | 0.848 | 0.826 | 0.837 | | | | 250 | 0.234 | 0.247 | 0.241 | | | | 125 | 0.105 | 0.109 | 0.107 | | |
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货期
3-5 working days