产品描述
SELL(L-selectin):SELL是一种细胞黏附分子,属于选择素家族,广泛表达于白细胞表面,参与白细胞与内皮细胞的黏附和转移。SELL通过与其配体GlyCAM-1和CD34结合,参与免疫细胞在淋巴结和炎症部位的定向迁移。SELL的表达受到多种因素的调控,如炎症因子、细胞因子、荷尔蒙等。SELL的异常表达与多种疾病状态相关,如癌症、自身免疫性疾病等。
华美生物所提供的Human L-Selectin ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的SELL,其灵敏度为0.195 ng/mL,检测范围为0.78 ng/mL-50 ng/mL。
别名
SELL; LNHR; LYAM1; L-selectin; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL; CD antigen CD62L
缩写
SELL
Uniprot No
P14151
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Stem Cells
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human L-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
90 |
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| Range % |
84-95 |
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| 1:10 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:20 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:40 |
Average % |
94 |
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| Range % |
86-101 |
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回收率
| The recovery of human L-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
86-94 |
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| EDTA plasma (n=4) |
92 |
85-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.498 |
2.662 |
2.580 |
2.409 |
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| 25 |
2.166 |
2.252 |
2.209 |
2.038 |
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| 12.5 |
1.651 |
1.669 |
1.660 |
1.489 |
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| 6.25 |
0.932 |
0.967 |
0.950 |
0.779 |
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| 3.12 |
0.550 |
0.514 |
0.532 |
0.361 |
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| 1.56 |
0.363 |
0.352 |
0.358 |
0.187 |
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| 0.78 |
0.265 |
0.284 |
0.275 |
0.104 |
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| 0 |
0.173 |
0.169 |
0.171 |
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货期
3-5 working days