产品描述

This Human S100A1 ELISA Kit was designed for the quantitative measurement of Human S100A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

Bpb ELISA Kit; NEF ELISA Kit; Protein S100-A1 ELISA Kit; S-100 protein alpha chain ELISA Kit; S-100 protein subunit alpha ELISA Kit; S100 alpha ELISA Kit; S100 beta ELISA Kit; S100 calcium binding protein A1 ELISA Kit; S100 calcium binding protein B ELISA Kit; S100 calcium-binding protein A1 ELISA Kit; S100 protein alpha polypeptide ELISA Kit; S100A ELISA Kit; s100a1 ELISA Kit; S100B ELISA Kit; S100beta ELISA Kit; S10A1_HUMAN ELISA Kit

缩写

S100A1

Uniprot No

P23297

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human S-100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		90-96
1:2		Average %		102
		Range %		97-107
1:4		Average %		87
		Range %		82-90
1:8		Average %		100
		Range %		97-103

回收率

The recovery of human S-100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		97		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.401 2.438 2.420 2.284 5 1.892 1.871 1.882 1.746 2.5 1.212 1.234 1.223 1.087 1.25 0.661 0.698 0.680 0.544 0.625 0.364 0.384 0.374 0.238 0.312 0.283 0.261 0.272 0.136 0.156 0.206 0.213 0.210 0.074 0 0.135 0.137 0.136

货期

3-5 working days