产品描述

This Guinea pig TNF ELISA Kit was designed for the quantitative measurement of Guinea pig TNF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

别名

TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit

缩写

TNF

Uniprot No

P51435

灵敏度

0.78 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		90-100
1:2		Average %		94
		Range %		89-98
1:4		Average %		92
		Range %		87-96
1:8		Average %		93
		Range %		89-100

回收率

The recovery of guinea pig TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-97
EDTA plasma (n=4)		97		92-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 200 2.350 2.376 2.363 2.200 100 1.884 1.902 1.893 1.730 50 1.368 1.379 1.374 1.211 25 0.961 0.973 0.967 0.804 12.5 0.664 0.681 0.673 0.510 6.25 0.475 0.486 0.481 0.318 3.12 0.305 0.309 0.307 0.144 0 0.162 0.164 0.163

货期

3-5 working days