产品描述
This Rat TSP-1 ELISA Kit was designed for the quantitative measurement of Rat TSP-1 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.
缩写
TSP-1
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TSP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:100 | Average % | 97 | |
| Range % | 94-100 | |
| 1:200 | Average % | 89 | |
| Range % | 84-92 | |
| 1:400 | Average % | 104 | |
| Range % | 100-108 | |
| 1:800 | Average % | 96 | |
| Range % | 93-99 | |
回收率
| The recovery of rat TSP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 82-91 | |
| EDTA plasma (n=4) | 94 | 90-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.405 | 2.487 | 2.446 | 2.272 | | | 200 | 1.992 | 1.972 | 1.982 | 1.808 | | | 100 | 1.583 | 1.598 | 1.591 | 1.417 | | | 50 | 1.131 | 1.168 | 1.150 | 0.976 | | | 25 | 0.748 | 0.728 | 0.738 | 0.564 | | | 12.5 | 0.498 | 0.462 | 0.480 | 0.306 | | | 6.25 | 0.335 | 0.314 | 0.325 | 0.151 | | | 0 | 0.173 | 0.175 | 0.174 | | |
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货期
3-5 working days