产品描述

The human COX-2 ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure human COX-2 in serum, plasma, cell lysates, or tissue homogenates. It is based on the Sandwich-ELISA mechanism. COX-2 in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated COX-2 antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the COX-2 bound in the initial step. The COX-2 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

COX-2 (PTGS2) catalyzes the conversion of arachidonic acid to prostaglandins and is an inducible type of cyclo-oxygenase. It is primarily responsible for inflammation, pain, and fever. COX-2 overexpression has been found in a variety of malignancies, including colorectal, breast, pancreatic, and lung cancers, and has been linked to a bad prognosis. In response to synaptic excitement, neuronal COX-2 is activated, releasing PGE2, the most common COX-2 metabolite in the brain, which increases glutamate release and neuronal firing in a retrograde manner. COX-2 is also involved in the synthesis of new endocannabinoids from 2-arachidonoyl-glycerol in order to control their activities at presynaptic terminals.

别名

COX 2 ELISA Kit; COX-2 ELISA Kit; COX2 ELISA Kit; Cyclooxygenase 2 ELISA Kit; Cyclooxygenase 2b ELISA Kit; Cyclooxygenase ELISA Kit; Cyclooxygenase-2 ELISA Kit; Cyclooxygenase2 ELISA Kit; EC 1.14.99.1 ELISA Kit; fj02a10 ELISA Kit; Glucocorticoid-regulated inflammatory cyclooxygenase ELISA Kit; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase ELISA Kit; GRIPGHS ELISA Kit; hCox 2 ELISA Kit; Macrophage activation-associated marker protein P71/73 ELISA Kit; OTTHUMP00000033524 ELISA Kit; PES-2 ELISA Kit; PGG/HS ELISA Kit; PGH synthase 2 ELISA Kit; PGH2_HUMAN ELISA Kit; PGHS 2 ELISA Kit; PGHS-2 ELISA Kit; PGHS2 ELISA Kit; PHS 2 ELISA Kit; PHS II ELISA Kit; PHS2 ELISA Kit; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) ELISA Kit; Prostaglandin endoperoxide synthase 2 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; Prostaglandin G/H synthase 2 precursor ELISA Kit; Prostaglandin G/H synthase and cyclooxygenase ELISA Kit; Prostaglandin G/H synthase ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit; PTGS2 ELISA Kit; ptgs2a ELISA Kit; TIS10 ELISA Kit; TIS10 protein ELISA Kit; unp1239 ELISA Kit; wu:fj02a10 ELISA Kit

缩写

PTGS2

Uniprot No

P35354

灵敏度

0.31 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		98
		Range %		94-102
1:2		Average %		95
		Range %		91-99
1:4		Average %		90
		Range %		85-94
1:8		Average %		84
		Range %		80-88

回收率

The recovery of human COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		87-100
EDTA plasma (n=4)		88		82-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 80 2.701 2.652 2.677 2.583 40 2.092 1.949 2.021 1.927 20 1.301 1.228 1.265 1.171 10 0.587 0.571 0.579 0.485 5 0.357 0.374 0.366 0.272 2.5 0.207 0.213 0.210 0.116 1.25 0.152 0.161 0.157 0.063 0 0.092 0.096 0.094

货期

3-5 working days