产品描述
The Rat soluble E-selectin (sE-selectin) ELISA Kit is a powerful tool for immunology researchers to measure the levels of sE-selectin in a variety of sample types from Rattus norvegicus (Rat).
This ELISA kit has a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL, providing accurate and reliable results for even low levels of E-selectin. The assay time is 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.
The sE-selectin ELISA Kit utilizes a sandwich assay principle, where the E-selectin antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.
This ELISA kit is suitable for a range of sample types, including serum, plasma, cell culture supernates, and tissue homogenates, making it a versatile solution for researchers in the field of immunology. It has been cited in over 9 publications, highlighting its reliability and utility in research.
别名
Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
缩写
SELE
Uniprot No
P98105
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 92 | |
| Range % | 88-108 | |
| 1:10 | Average % | 94 | |
| Range % | 91-98 | |
| 1:20 | Average % | 96 | |
| Range % | 89-100 | |
| 1:40 | Average % | 84 | |
| Range % | 80-88 | |
回收率
| The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 99 | 93-105 | |
| EDTA plasma (n=4) | 94 | 90-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 5000 | 2.746 | 2.645 | 2.696 | 2.542 | | | 2500 | 1.976 | 1.875 | 1.926 | 1.772 | | | 1250 | 1.228 | 1.204 | 1.216 | 1.062 | | | 625 | 0.782 | 0.761 | 0.772 | 0.618 | | | 312 | 0.522 | 0.511 | 0.517 | 0.363 | | | 156 | 0.363 | 0.352 | 0.358 | 0.204 | | | 78 | 0.290 | 0.285 | 0.288 | 0.134 | | | 0 | 0.154 | 0.153 | 0.154 | | |
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货期
3-5 working days
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