产品描述
This Human SFTPB ELISA Kit was designed for the quantitative measurement of Human SFTPB protein in serum, plasma, urine, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
SFTPB; SFTP3; Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe
缩写
SFTPB
Uniprot No
P07988
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 89 | |
| Range % | 83-95 | |
| 1:200 | Average % | 93 | |
| Range % | 87-100 | |
| 1:400 | Average % | 88 | |
| Range % | 85-91 | |
| 1:800 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of human SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-99 | |
| EDTA plasma (n=4) | 96 | 89-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.453 | 2.563 | 2.508 | 2.347 | | | 5 | 2.228 | 2.261 | 2.245 | 2.084 | | | 2.5 | 1.795 | 1.801 | 1.798 | 1.637 | | | 1.25 | 1.267 | 1.297 | 1.282 | 1.121 | | | 0.625 | 0.811 | 0.834 | 0.823 | 0.662 | | | 0.312 | 0.532 | 0.575 | 0.554 | 0.393 | | | 0.156 | 0.362 | 0.371 | 0.367 | 0.206 | | | 0 | 0.160 | 0.162 | 0.161 | | |
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货期
3-5 working days