产品描述

This Human S100A4 ELISA Kit was designed for the quantitative measurement of Human S100A4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.225 ng/mL.

别名

18A2 ELISA Kit; 42A ELISA Kit; calcium Placental protein ELISA Kit; Calvasculin ELISA Kit; CAPL ELISA Kit; Fibroblast specific protein 1 (FSP1) ELISA Kit; Fibroblast specific protein 1 ELISA Kit; Fibroblast specific protein ELISA Kit; FSP1 ELISA Kit; Leukemia multidrug resistance associated protein ELISA Kit; Malignant transformation suppression 1 (MTS1) ELISA Kit; Malignant transformation suppression 1 ELISA Kit; Metastasin ELISA Kit; MTS1 ELISA Kit; OTTHUMP00000015467 ELISA Kit; OTTHUMP00000015468 ELISA Kit; P9KA ELISA Kit; PEL98 ELISA Kit; Placental calcium-binding protein ELISA Kit; Protein Mts1 ELISA Kit; Protein S100 A4 ELISA Kit; Protein S100-A4 ELISA Kit; S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) ELISA Kit; S100 calcium binding protein A4 ELISA Kit; S100 calcium-binding protein A4 ELISA Kit; S100a4 ELISA Kit; S10A4_HUMAN ELISA Kit

缩写

S100A4

Uniprot No

P26447

灵敏度

0.225 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-96
1:2		Average %		93
		Range %		82-101
1:4		Average %		97
		Range %		93-104
1:8		Average %		93
		Range %		86-98

回收率

The recovery of human S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		92-105
EDTA plasma (n=4)		100		95-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   60 2.654 2.587 2.621 2.471   30 2.216 2.115 2.166 2.016   15 1.369 1.406 1.388 1.238   7.5 0.689 0.715 0.702 0.552   3.75 0.385 0.367 0.376 0.226   1.8 0.288 0.279 0.284 0.134   0.9 0.216 0.206 0.211 0.061   0 0.156 0.144 0.150

货期

3-5 working days