产品描述
PRL(Prolactin)PRL是一种垂体前叶激素,主要作用于哺乳动物的乳腺和生殖系统。它能够促进乳腺发育和乳汁分泌,同时也调节生殖系统的功能。此外,PRL还参与调节免疫系统、代谢和行为等生理过程,其异常水平与多种疾病的发生相关。
华美生物所提供的Human Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆、细胞裂解液样本中的PRL,其灵敏度为50 μIU/mL,检测范围为40 μIU/mL-2000 μIU/mL。
别名
PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
缩写
PRL
Uniprot No
P01236
灵敏度
50 μIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
86-97 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-100 |
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| 1:4 |
Average % |
95 |
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| Range % |
92-99 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-98 |
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回收率
| The recovery of human PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-102 |
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| EDTA plasma (n=4) |
97 |
92-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.186 |
2.105 |
2.146 |
2.115 |
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| 800 |
1.201 |
1.197 |
1.199 |
1.168 |
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| 300 |
0.622 |
0.619 |
0.621 |
0.590 |
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| 100 |
0.296 |
0.302 |
0.299 |
0.268 |
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| 40 |
0.145 |
0.148 |
0.147 |
0.116 |
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| 0 |
0.032 |
0.030 |
0.031 |
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货期
3-5 working days