产品描述

别名

NEF ELISA Kit; Protein S100 B ELISA Kit; Protein S100-B ELISA Kit; S 100 calcium binding protein beta chain ELISA Kit; S 100 protein beta chain ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 ELISA Kit; S100 calcium binding protein beta (neural) ELISA Kit; S100 calcium-binding protein B ELISA Kit; S100 protein beta chain ELISA Kit; S100B ELISA Kit; S100B_HUMAN ELISA Kit; S100beta ELISA Kit

缩写

S100B

Uniprot No

P04271

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-95
1:2		Average %		89
		Range %		85-94
1:4		Average %		95
		Range %		92-100
1:8		Average %		90
		Range %		85-98

回收率

The recovery of human S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		85-99
EDTA plasma (n=4)		96		92-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   5000 2.037 1.902 1.970 1.809   2500 1.585 1.583 1.584 1.423   1250 1.184 1.156 1.170 1.009   625 0.821 0.852 0.837 0.676   312 0.595 0.581 0.588 0.427   156 0.372 0.380 0.376 0.215   78 0.249 0.234 0.242 0.081   0 0.164 0.158 0.161

货期

3-5 working days