产品描述
The Rat placenta growth factor(PLGF)ELISA Kit allows for the in vitro quantitative determination of PLGF concentrations in serum, plasma, or tissue homogenates. The detection mechanism of this kit is based on the Sandwich-ELISA technique. PLGFin the sample is bound to the anti-rat PLGFantibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled PLGFantibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with PLGFcontent in the sample. This kit exclusively recognizes rat PLGF. The kit has been quality controlled with high sensitivity, strong specificity, good linearity, precision of less than 10%, recovery of 89%-98%, and high lot-to-lot consistency. Refer to the product manual for more information.
PLGF is a member of the VEGF family and is mainly expressed in the placenta. It is a crucial molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. Specifically, PLGF plays a role in trophoblast growth and differentiation. In human atherosclerotic lesions, PLGF expression is linked to plaque inflammation and neovascular growth. PLGF is also a potential biomarker for pre-eclampsia, a pregnancy complication characterized by hypertension and proteinuria in pregnancy.
别名
Pgf ELISA Kit; Plgf ELISA Kit; Placenta growth factor ELISA Kit; PlGF ELISA Kit
缩写
PGF
Uniprot No
Q63434
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PLGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-95 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-97 |
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| 1:8 |
Average % |
99 |
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| Range % |
93-102 |
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回收率
| The recovery of rat PLGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-97 |
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| EDTA plasma (n=4) |
94 |
91-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.246 |
2.234 |
2.240 |
2.085 |
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| 100 |
1.472 |
1.456 |
1.464 |
1.309 |
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| 50 |
0.901 |
0.914 |
0.908 |
0.753 |
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| 25 |
0.639 |
0.649 |
0.644 |
0.489 |
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| 12.5 |
0.364 |
0.389 |
0.377 |
0.222 |
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| 6.25 |
0.300 |
0.308 |
0.304 |
0.149 |
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| 3.12 |
0.252 |
0.242 |
0.247 |
0.092 |
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| 0 |
0.156 |
0.154 |
0.155 |
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货期
3-5 working days
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