产品描述
TNFRSF1B,全名为肿瘤坏死因子受体家族成员 1B(TNF receptor superfamily member 1B),也称为TNFR2。TNFR2的分布比TNFR1更为局限,主要存在于免疫细胞表面,其信号通路与TNFR1类似,但与细胞增殖和凋亡的调节有关的分子和通路不同。TNFR2的调节和作用机制与TNFR1存在着复杂的相互影响和调节。
华美生物所提供的Mouse Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的TNFRSF1B,其灵敏度为1.6 pg/mL,检测范围为7.8125 pg/mL-500 pg/mL。
别名
Tnfrsf1b; Tnfr-2; Tnfr2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD antigen CD120b
缩写
TNFRSF1B
Uniprot No
P25119
灵敏度
1.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFsR-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
91 |
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| Range % |
86-95 |
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| 1:40 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:80 |
Average % |
90 |
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| Range % |
87-98 |
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| 1:160 |
Average % |
92 |
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| Range % |
85-96 |
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回收率
| The recovery of mouse TNFsR-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-97 |
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| EDTA plasma (n=4) |
98 |
95-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.858 |
2.911 |
2.885 |
2.777 |
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| 250 |
2.360 |
2.250 |
2.305 |
2.197 |
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| 125 |
1.485 |
1.455 |
1.470 |
1.362 |
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| 62.5 |
0.785 |
0.812 |
0.799 |
0.691 |
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| 31.25 |
0.482 |
0.521 |
0.502 |
0.394 |
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| 15.625 |
0.270 |
0.263 |
0.267 |
0.159 |
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| 7.8125 |
0.189 |
0.187 |
0.188 |
0.080 |
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| 0 |
0.104 |
0.112 |
0.108 |
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货期
3-5 working days