产品描述

This Human RUNX2 ELISA Kit was designed for the quantitative measurement of Human RUNX2 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

Acute myeloid leukemia 3 protein ELISA Kit; Alpha subunit 1 ELISA Kit; AML3 ELISA Kit; CBF alpha 1 ELISA Kit; CBF-alpha-1 ELISA Kit; CBFA1 ELISA Kit; CCD ELISA Kit; CCD1 ELISA Kit; Cleidocranial dysplasia 1 ELISA Kit; Core binding factor ELISA Kit; Core binding factor runt domain alpha subunit 1 ELISA Kit; Core binding factor subunit alpha 1 ELISA Kit; Core-binding factor subunit alpha-1 ELISA Kit; MGC120022 ELISA Kit; MGC120023 ELISA Kit; Oncogene AML 3 ELISA Kit; Oncogene AML-3 ELISA Kit; OSF 2 ELISA Kit; OSF-2 ELISA Kit; OSF2 ELISA Kit; Osteoblast specific transcription factor 2 ELISA Kit; Osteoblast-specific transcription factor 2 ELISA Kit; OTTHUMP00000016533 ELISA Kit; PEA2 alpha A ELISA Kit; PEA2-alpha A ELISA Kit; PEA2aA ELISA Kit; PEBP2 alpha A ELISA Kit; PEBP2-alpha A ELISA Kit; PEBP2A1 ELISA Kit; PEBP2A2 ELISA Kit; PEBP2aA ELISA Kit; PEBP2aA1 ELISA Kit; Polyomavirus enhancer binding protein 2 alpha A subunit ELISA Kit; Polyomavirus enhancer-binding protein 2 alpha A subunit ELISA Kit; Runt domain ELISA Kit; Runt related transcription factor 2 ELISA Kit; Runt-related transcription factor 2 ELISA Kit; RUNX2 ELISA Kit; RUNX2_HUMAN ELISA Kit; SL3 3 enhancer factor 1 alpha A subunit ELISA Kit; SL3-3 enhancer factor 1 alpha A subunit ELISA Kit; SL3/AKV core binding factor alpha A subunit ELISA Kit; SL3/AKV core-binding factor alpha A subunit ELISA Kit

缩写

RUNX2

Uniprot No

Q13950

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CBFA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:200		Average %		91
		Range %		86-95
1:400		Average %		102
		Range %		97-107
1:800		Average %		91
		Range %		85-97
1:1600		Average %		97
		Range %		91-103

回收率

The recovery of human CBFA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		97		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 2000 1.957 1.999 1.978 1.887 1000 1.567 1.603 1.585 1.494 500 1.106 1.101 1.104 1.013 250 0.719 0.732 0.726 0.635 125 0.407 0.412 0.410 0.319 62.5 0.283 0.298 0.291 0.200 31.25 0.164 0.165 0.165 0.074 0 0.090 0.092 0.091

货期

3-5 working days