产品描述
TNF(肿瘤坏死因子)是一种细胞因子,由多种免疫细胞产生,包括巨噬细胞、T细胞和B细胞。TNF参与了机体的免疫调节、炎症反应等多种生理和病理过程,与多种疾病的发生和发展有关,如肿瘤、自身免疫性疾病、感染性疾病等。TNF也是一种炎症标志物,可以用于评估机体的炎症反应情况。
华美生物所提供的Rat TNF-α ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆样本中的TNF,其灵敏度为1.56 pg/mL,检测范围为6.25 pg/mL-400 pg/mL。
别名
Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
缩写
TNF
Uniprot No
P16599
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
87-96 |
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| 1:2 |
Average % |
99 |
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| Range % |
95-104 |
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| 1:4 |
Average % |
101 |
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| Range % |
97-105 |
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| 1:8 |
Average % |
98 |
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| Range % |
93-107 |
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回收率
| The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
80-92 |
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| EDTA plasma (n=4) |
99 |
95-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.037 |
2.067 |
2.052 |
1.871 |
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| 200 |
1.445 |
1.489 |
1.467 |
1.286 |
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| 100 |
1.017 |
1.034 |
1.026 |
0.845 |
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| 50 |
0.655 |
0.677 |
0.666 |
0.485 |
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| 25 |
0.411 |
0.438 |
0.425 |
0.244 |
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| 12.5 |
0.351 |
0.378 |
0.365 |
0.184 |
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| 6.25 |
0.297 |
0.282 |
0.290 |
0.109 |
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| 0 |
0.183 |
0.179 |
0.181 |
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货期
3-5 working days