产品描述

The human p53 ELISA Kit is used to quantitatively measure human p53 concentrations in many different samples, including serum, cell culture supernates, urine, cerebrospinal fluid (CSF), tissue homogenates, or cell lysates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of p53 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

The p53 protein is encoded by the gene TP53 and plays a prominent role in tumor suppression through its ability to induce apoptosis, cell cycle arrest, and senescence of pre-cancerous cells. It has recently been proved that p53's function in the modulation of genes involved in metabolism and ferroptosis has mediated in its capacity to inhibit tumor development. p53 can activate autophagy, which also facilitates tumor suppression. It also regulates many metabolic pathways of glucose, lipid, and amino acid metabolism, enabling cells to adapt to and survive under mild metabolic stress.

别名

Antigen NY-CO-13 ELISA Kit; BCC7 ELISA Kit; Cellular tumor antigen p53 ELISA Kit; FLJ92943 ELISA Kit; LFS1 ELISA Kit; Mutant tumor protein 53 ELISA Kit; p53 ELISA Kit; p53 tumor suppressor ELISA Kit; P53_HUMAN ELISA Kit; Phosphoprotein p53 ELISA Kit; Tp53 ELISA Kit; Transformation related protein 53 ELISA Kit; TRP53 ELISA Kit; tumor antigen p55 ELISA Kit; Tumor protein 53 ELISA Kit; Tumor protein p53 ELISA Kit; Tumor suppressor p53 ELISA Kit

缩写

TP53

Uniprot No

P04637

灵敏度

2.34 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human p53/TP53 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		80-110
1:2		Average %		97
		Range %		91-105
1:4		Average %		97
		Range %		92-105
1:8		Average %		91
		Range %		86-98

回收率

The recovery of human p53/TP53 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		89-110

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 600 2.623 2.676 2.650 2.520 300 2.213 2.286 2.250 2.120 150 1.678 1.698 1.688 1.558 75 1.059 1.089 1.074 0.944 37.5 0.648 0.689 0.669 0.539 18.7 0.441 0.480 0.461 0.331 9.38 0.298 0.300 0.299 0.169 0 0.133 0.127 0.130

货期

3-5 working days