产品描述

This Human PYCARD ELISA Kit was designed for the quantitative measurement of Human PYCARD protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

Apoptosis associated speck like protein containing a CARD ELISA Kit; Apoptosis-associated speck-like protein containing a CARD ELISA Kit; ASC ELISA Kit; ASC_HUMAN ELISA Kit; CARD 5 ELISA Kit; CARD5 ELISA Kit; Caspase recruitment domain containing protein 5 ELISA Kit; Caspase recruitment domain protein 5 ELISA Kit; Caspase recruitment domain-containing protein 5 ELISA Kit; hASC ELISA Kit; MGC10332 ELISA Kit; PYCARD ELISA Kit; PYD and CARD domain containing ELISA Kit; PYD and CARD domain containing protein ELISA Kit; PYD and CARD domain-containing protein ELISA Kit; Target of methylation induced silencing 1 ELISA Kit; Target of methylation-induced silencing 1 ELISA Kit; TMS 1 ELISA Kit; TMS ELISA Kit; TMS1 ELISA Kit

缩写

PYCARD

Uniprot No

Q9ULZ3

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PYCARD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		90-102
1:2		Average %		90
		Range %		85-99
1:4		Average %		98
		Range %		92-106
1:8		Average %		92
		Range %		86-100

回收率

The recovery of human PYCARD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-98
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1000 1.834 1.867 1.851 1.713   500 1.517 1.541 1.529 1.391   250 1.213 1.224 1.219 1.081   125 0.892 0.902 0.897 0.759   62.5 0.561 0.578 0.570 0.432   31.2 0.345 0.352 0.349 0.211   15.6 0.256 0.266 0.261 0.123   0 0.137 0.139 0.138

货期

3-5 working days