产品描述
This Rat TN-C ELISA Kit was designed for the quantitative measurement of Rat TN-C protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.5 ng/mL-160 ng/mL and the sensitivity is 0.625 ng/mL.
缩写
TN-C
灵敏度
0.625 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TN-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:4 |
Average % |
106 |
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| Range % |
101-113 |
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| 1:8 |
Average % |
93 |
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| Range % |
89-98 |
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回收率
| The recovery of rat TN-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
88 |
84-92 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 160 |
2.417 |
2.502 |
2.460 |
2.301 |
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| 80 |
2.102 |
2.173 |
2.138 |
1.979 |
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| 40 |
1.700 |
1.615 |
1.658 |
1.499 |
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| 20 |
1.085 |
1.049 |
1.067 |
0.908 |
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| 10 |
0.671 |
0.684 |
0.678 |
0.519 |
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| 5 |
0.424 |
0.437 |
0.431 |
0.272 |
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| 2.5 |
0.293 |
0.281 |
0.287 |
0.128 |
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| 0 |
0.162 |
0.156 |
0.159 |
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货期
3-5 working days