产品描述
The human progranulin (PGRN) ELISA Kit is used to quantitatively measure PGRN concentrations in human serum, plasma, or cell culture supernates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of PGRN in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
PGRN is a secreted growth factor that is involved in the modulation of multiple processes through itself or its conversion to granulins, including cell proliferation, survival, inflammation, wound healing, and tumorigenesis. It plays a major role in regulating lysosomal function and microglial responses in the central nervous system (CNS). In the brain, PGRN is mainly expressed by neurons and microglia and is involved in neuronal survival, neurite outgrowth, and synaptogenesis. It is a key regulator of neuroinflammation. Heterozygous mutations in PGRN lead to PGRN haploinsufficiency, which is one of the main causes of frontotemporal lobar degeneration (FTLD) related to the accumulation of TDP-43 inclusions.
缩写
PGRN
灵敏度
1.17 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 103 | |
| Range % | 97-110 | |
| 1:2 | Average % | 97 | |
| Range % | 92-101 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of human PGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
89 |
84-93 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 300 |
2.824 |
2.952 |
2.888 |
2.717 |
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| 150 |
2.332 |
2.176 |
2.254 |
2.083 |
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| 75 |
1.488 |
1.404 |
1.446 |
1.275 |
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| 37.5 |
0.856 |
0.839 |
0.848 |
0.677 |
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| 18.75 |
0.495 |
0.474 |
0.485 |
0.314 |
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| 9.4 |
0.335 |
0.359 |
0.347 |
0.176 |
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| 4.7 |
0.221 |
0.229 |
0.225 |
0.054 |
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| 0 |
0.164 |
0.177 |
0.171 |
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货期
3-5 working days
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