产品描述
PLG,也称为纤溶酶原,是人体内的一种蛋白质,它是纤溶系统的重要成分,能够在机体受到创伤或炎症时促进血凝块形成和溶解,起到了非常重要的保护作用。
华美生物所提供的Rabbit Plasminogen,Plg ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测兔血清、血浆、组织匀浆样本中的PLG,其灵敏度为15.6 ng/mL,检测范围为62.5 ng/mL-4000 ng/mL。
别名
Plasmin ELISA Kit; Plasmin heavy chain A ELISA Kit; Plasmin light chain B ELISA Kit; Plasminogen ELISA Kit; PLG ELISA Kit; PLMN_HUMAN ELISA Kit
缩写
PLG
Uniprot No
P00747
灵敏度
15.6 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Plg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
86-103 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
88-97 |
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| 1:8 |
Average % |
90 |
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| Range % |
83-100 |
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回收率
| The recovery of rabbit Plg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
88-102 |
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| EDTA plasma (n=4) |
97 |
83-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 4000 |
0.113 |
0.115 |
0.114 |
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| 2000 |
0.216 |
0.227 |
0.222 |
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| 1000 |
0.467 |
0.472 |
0.470 |
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| 500 |
0.785 |
0.792 |
0.789 |
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| 250 |
1.218 |
1.220 |
1.219 |
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| 125 |
1.682 |
1.693 |
1.688 |
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| 62.5 |
2.159 |
2.172 |
2.166 |
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| 0 |
2.600 |
2.629 |
2.615 |
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货期
3-5 working days