产品描述
This Mouse PDGF-BB ELISA Kit was designed for the quantitative measurement of Mouse PDGF-BB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 ng/mL-12 ng/mL and the sensitivity is 0.047 ng/mL.
缩写
PDGF-BB
灵敏度
0.047 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 82-88 | |
| 1:2 | Average % | 94 | |
| Range % | 89-98 | |
| 1:4 | Average % | 104 | |
| Range % | 97-109 | |
| 1:8 | Average % | 99 | |
| Range % | 93-105 | |
回收率
| The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-101 | |
| EDTA plasma (n=4) | 89 | 82-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 12 | 2.389 | 2.501 | 2.445 | 2.281 | | | 6 | 1.445 | 1.529 | 1.487 | 1.323 | | | 3 | 0.905 | 0.984 | 0.945 | 0.781 | | | 1.5 | 0.656 | 0.633 | 0.645 | 0.481 | | | 0.75 | 0.345 | 0.349 | 0.347 | 0.183 | | | 0.37 | 0.287 | 0.265 | 0.276 | 0.112 | | | 0.18 | 0.223 | 0.218 | 0.221 | 0.057 | | | 0 | 0.159 | 0.169 | 0.164 | | |
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货期
3-5 working days