别名

ALK 1 ELISA Kit; ALK1 ELISA Kit; ALP ELISA Kit; Antileukoprotease ELISA Kit; Antileukoproteinase 1 ELISA Kit; Antileukoproteinase ELISA Kit; Antileukoproteinase1 ELISA Kit; BLPI ELISA Kit; Human seminal protease inhibitor ELISA Kit; HUSI 1 ELISA Kit; HUSI ELISA Kit; HUSI I ELISA Kit; HUSI-1 ELISA Kit; MPI ELISA Kit; Mucus proteinase inhibitor ELISA Kit; Protease inhibitor WAP4 ELISA Kit; Secretory leukocyte peptidase inhibitor ELISA Kit; Secretory leukocyte protease inhibitor ELISA Kit; Seminal proteinase inhibitor ELISA Kit; SLPI ELISA Kit; SLPI_HUMAN ELISA Kit; WAP four disulfide core domain protein 4 ELISA Kit; WAP four-disulfide core domain protein 4 ELISA Kit; WAP4 ELISA Kit; WFDC4 ELISA Kit

缩写

SLPI

Uniprot No

P03973

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SLPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		96
		Range %		91-102
1:200		Average %		92
		Range %		85-98
1:400		Average %		89
		Range %		83-94
1:800		Average %		91
		Range %		87-96

回收率

The recovery of human SLPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		93-102
EDTA plasma (n=4)		86		81-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     2000 2.637 2.531 2.584 2.490     1000 2.124 2.205 2.165 2.071     500 1.454 1.557 1.506 1.412     250 0.936 0.923 0.930 0.836     125 0.448 0.478 0.463 0.369     62.5 0.283 0.274 0.279 0.185     31.25 0.156 0.163 0.160 0.066     0 0.093 0.095 0.094

货期

3-5 working days