产品描述

This Human SFTPD ELISA Kit was designed for the quantitative measurement of Human SFTPD protein in serum, plasma, bronchoalveolar lavage fluid, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL.

别名

COLEC 7 ELISA Kit; COLEC7 ELISA Kit; Collectin-7 ELISA Kit; Collectin7 ELISA Kit; Lung surfactant protein D ELISA Kit; PSP D ELISA Kit; PSP-D ELISA Kit; PSP-D Surfactant protein D ELISA Kit; PSPD ELISA Kit; Pulmonary surfactant apoprotein ELISA Kit; Pulmonary surfactant associated protein D ELISA Kit; Pulmonary surfactant associated protein PSP-D ELISA Kit; Pulmonary surfactant-associated protein D ELISA Kit; SFTP 4 ELISA Kit; SFTP4 ELISA Kit; SFTPD ELISA Kit; SFTPD_HUMAN ELISA Kit; SP D ELISA Kit; SP-D ELISA Kit; Surfactant associated protein pulmonary 4 ELISA Kit; Surfactant protein D ELISA Kit; Surfactant pulmonary associated protein D ELISA Kit

缩写

SFTPD

Uniprot No

P35247

灵敏度

0.31 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-94
1:2		Average %		96
		Range %		91-100
1:4		Average %		98
		Range %		92-108
1:8		Average %		92
		Range %		86-97

回收率

The recovery of human SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-97
EDTA plasma (n=4)		98		90-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 80 2.198 2.068 2.133 2.042 40 1.457 1.378 1.418 1.327 20 0.925 0.884 0.905 0.814 10 0.600 0.578 0.589 0.498 5 0.410 0.393 0.402 0.311 2.5 0.255 0.251 0.253 0.162 1.25 0.136 0.134 0.135 0.044 0 0.092 0.090 0.091

货期

3-5 working days