产品描述

This Human TNFRSF25 ELISA Kit was designed for the quantitative measurement of Human TNFRSF25 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.24 ng/mL-15 ng/mL and the sensitivity is 0.06 ng/mL.

别名

Apo 3 ELISA Kit; Apo-3 ELISA Kit; Apo3 ELISA Kit; Apoptosis inducing receptor AIR ELISA Kit; Apoptosis inducing receptor ELISA Kit; Apoptosis mediating receptor ELISA Kit; Apoptosis mediating receptor DR 3 ELISA Kit; Apoptosis mediating receptor DR3 ELISA Kit; Apoptosis mediating receptor TRAMP ELISA Kit; Apoptosis-inducing receptor AIR ELISA Kit; Apoptosis-mediating receptor DR3 ELISA Kit; Apoptosis-mediating receptor TRAMP ELISA Kit; DDR 3 ELISA Kit; DDR3 ELISA Kit; Death domain receptor 3 ELISA Kit; Death domain receptor 3 soluble form ELISA Kit; Death receptor 3 ELISA Kit; Death receptor beta ELISA Kit; DR 3 ELISA Kit; DR3 ELISA Kit; LARD ELISA Kit; Lymphocyte associated receptor of death ELISA Kit; Lymphocyte-associated receptor of death ELISA Kit; Protein WSL ELISA Kit; Protein WSL-1 ELISA Kit; TNF receptor superfamily member 25 ELISA Kit; TNFR25 ELISA Kit; TNFRSF 12 ELISA Kit; TNFRSF 25 ELISA Kit; TNFRSF12 ELISA Kit; TNFRSF12, formerly ELISA Kit; TNFRSF25 ELISA Kit; TNR25_HUMAN ELISA Kit; TR 3 ELISA Kit; TR3 ELISA Kit; TRAMP ELISA Kit; Translocating chain association membrane protein ELISA Kit; Tumor necrosis factor receptor superfamily member 12 ELISA Kit; Tumor necrosis factor receptor superfamily member 25 ELISA Kit; Tumor necrosis factor receptor superfamily, member 12 (translocating chain association membrane protein) ELISA Kit; Tumor necrosis factor receptor superfamily, member 12, formerly ELISA Kit; WSL 1 ELISA Kit; WSL ELISA Kit; WSL LR ELISA Kit; WSL protein ELISA Kit; WSL1 ELISA Kit; WSL1 protein ELISA Kit; WSLLR ELISA Kit

缩写

TNFRSF25

Uniprot No

Q93038

灵敏度

0.06 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TNFRSF25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		85-93
1:2		Average %		94
		Range %		91-99
1:4		Average %		84
		Range %		81-89
1:8		Average %		90
		Range %		87-93

回收率

The recovery of human TNFRSF25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		83		80-86
EDTA plasma (n=4)		91		88-95

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 15 2.464 2.498 2.481 2.321 7.5 1.696 1.668 1.682 1.522 3.75 1.101 1.135 1.118 0.958 1.88 0.712 0.732 0.722 0.562 0.94 0.513 0.536 0.525 0.365 0.47 0.398 0.387 0.393 0.233 0.24 0.297 0.276 0.287 0.127 0 0.161 0.159 0.160

货期

3-5 working days