产品描述
This Rat PECAM1 ELISA Kit was designed for the quantitative measurement of Rat PECAM1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
别名
Pecam1 ELISA Kit; Pecam ELISA Kit; Platelet endothelial cell adhesion molecule ELISA Kit; PECAM-1 ELISA Kit; CD antigen CD31 ELISA Kit
缩写
PECAM1
Uniprot No
Q3SWT0
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
86-95 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-107 |
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| 1:4 |
Average % |
91 |
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| Range % |
85-97 |
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| 1:8 |
Average % |
97 |
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| Range % |
91-103 |
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回收率
| The recovery of rat sPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.468 |
2.554 |
2.511 |
2.372 |
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| 100 |
1.656 |
1.732 |
1.694 |
1.555 |
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| 50 |
1.033 |
1.067 |
1.050 |
0.911 |
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| 25 |
0.591 |
0.611 |
0.601 |
0.462 |
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| 12.5 |
0.380 |
0.398 |
0.389 |
0.250 |
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| 6.25 |
0.278 |
0.286 |
0.282 |
0.143 |
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| 3.12 |
0.203 |
0.207 |
0.205 |
0.066 |
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| 0 |
0.138 |
0.139 |
0.139 |
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货期
3-5 working days