别名
Sftpd ELISA Kit; Sftp4 ELISA Kit; Pulmonary surfactant-associated protein D ELISA Kit; PSP-D ELISA Kit; SP-D ELISA Kit; Lung surfactant protein D ELISA Kit
缩写
SP-D
Uniprot No
P50404
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 82-92 | |
| 1:2 | Average % | 95 | |
| Range % | 90-100 | |
| 1:4 | Average % | 97 | |
| Range % | 91-101 | |
| 1:8 | Average % | 93 | |
| Range % | 89-99 | |
回收率
| The recovery of mouse SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-95 | |
| EDTA plasma (n=4) | 98 | 92-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.135 | 2.052 | 2.094 | 1.923 | | | 50 | 1.762 | 1.676 | 1.719 | 1.548 | | | 25 | 1.317 | 1.234 | 1.276 | 1.105 | | | 12.5 | 0.844 | 0.839 | 0.842 | 0.671 | | | 6.25 | 0.569 | 0.527 | 0.548 | 0.377 | | | 3.12 | 0.336 | 0.319 | 0.328 | 0.157 | | | 1.56 | 0.211 | 0.207 | 0.209 | 0.038 | | | 0 | 0.173 | 0.169 | 0.171 | | |
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货期
3-5 working days